Soil microbiome in chronosequence of spoil heaps of Kursk Magnetic Anomaly

Ekaterina Ivanova; Elizaveta Pershina; Dina Karpova; Olga Rogova; Evgeny Abakumov; Evgeny Andronov

doi:10.21638/spbu03.2019.306

Authors

Ekaterina Ivanova Federal State Budget Scientific Institution “All-Russian Research Institute of Agricultural Microbiology”, Shosse Podbelskogo, 3, Saint Petersburg, 196608, Russian Federation; V. V. Dokuchaev Soil Institute, Pyzhyovskiy Lane, 7 (building 2), Moscow, 119017, Russian Federation; Agrophysical Research Institute, Grazhdanskii Pr., 14, Saint Petersburg, 195220, Russian Federation https://orcid.org/0000-0003-1589-9875
Elizaveta Pershina Federal State Budget Scientific Institution “All-Russian Research Institute of Agricultural Microbiology”, Shosse Podbelskogo, 3, Saint Petersburg, 196608, Russian Federation; Department of Applied Ecology, Faculty of Biology, Saint Petersburg State University, 16th Liniya V. O., 29, Saint Petersburg, 199178, Russian Federation https://orcid.org/0000-0003-0834-3211
Dina Karpova Lomonosov Moscow State University, 1–12 Leninskie Gory, Moscow, 119234, Russian Federation https://orcid.org/0000-0002-9892-9621
Olga Rogova V. V. Dokuchaev Soil Institute, Pyzhyovskiy Lane, 7 (building 2), Moscow, 119017, Russian Federation https://orcid.org/0000-0003-2908-0828
Evgeny Abakumov Department of Applied Ecology, Faculty of Biology, Saint Petersburg State University, 16th Liniya V. O., 29, Saint Petersburg, 199178, Russian Federation https://orcid.org/0000-0002-5248-9018
Evgeny Andronov Federal State Budget Scientific Institution “All-Russian Research Institute of Agricultural Microbiology”, Shosse Podbelskogo, 3, Saint Petersburg, 196608, Russian Federation; Department of Applied Ecology, Faculty of Biology, Saint Petersburg State University, 16th Liniya V. O., 29, Saint Petersburg, 199178, Russian Federation; V. V. Dokuchaev Soil Institute, Pyzhyovskiy Lane, 7 (building 2), Moscow, 119017, Russian Federation https://orcid.org/0000-0003-0437-9292

DOI:

https://doi.org/10.21638/spbu03.2019.306

Abstract

The physicochemical parameters, vegetation and biodiversity of microbiomes inhabiting 10-, 35- and 50-year-old embryonic soils (technozems) formed in the mining areas of the Kursk Magnetic Anomaly (Russia) were analyzed in the current study. Analysis of taxonomic diversity was carried out by 454-pyrosequencing of the V4 variable region of the 16S rRNA gene. All points of the chronosequence were characterized by microbiomes reliably differing in taxonomic composition. The older the dump, the higher the proportion of bacteria from the phyla Actinobacteria (mostly representatives of the Solirubrobacteriaceae family), Chloroflexi and Acidobacteria in the community. In 10-year-old dumps, bacteria from the phyla Proteobacteria, Gemmatimonadetes and Bacteroides prevailed. The most pronounced changes in the community structure at the early and middle stages of microbiological succession were demonstrated by bacteria from the genus Delftia, which may indicate their active role in the processes of soil formation in this ecosystem.

Keywords:

microbial communities, embryonic soils, 454-pyrosequencing, 16S rRNA, overburden rock dumps

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