Cross-regulation of <em>Arabidopsis</em> root growth by plant hormones auxins and ethylene

Alla Korobova; Anna Vasinskaya; Anastasia Kirpichnikova; Maria Shishova; Guzel Kudoyarova

doi:10.21638/spbu03.2018.404

Authors

Alla Korobova Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, Prospekt Oktyabrya, 69, Ufa, 450054, Russian Federation https://orcid.org/0000-0002-5461-3553
Anna Vasinskaya Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, Prospekt Oktyabrya, 69, Ufa, 450054, Russian Federation https://orcid.org/0000-0001-8169-6780
Anastasia Kirpichnikova Department of Plant Physiology and Biochemistry, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0001-5133-5175
Maria Shishova Department of Plant Physiology and Biochemistry, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0003-3657-2986
Guzel Kudoyarova Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, Prospekt Oktyabrya, 69, Ufa, 450054, Russian Federation https://orcid.org/0000-0001-6409-9976

DOI:

https://doi.org/10.21638/spbu03.2018.404

Abstract

We investigated the cross-talk between auxin (IAA) and ethylene in the control of root growth of Arabidopsis plants (Arabidopsis thaliana). The root growth of ethylene insensitive etr1-1 and auxin insensitive tir1 mutants under the effect of IAA and 1-methylcyclopropene (1-MCP, inhibitor of ethylene perception) was compared, respectively, with that of the wild type ecotype Columbia (Col-0). Roots of Col-0 were shorter than those of the etr1-1 mutant. The addition of IAA (5.7 × 10^–6 М) to the growth medium led to 20 % root shortening in Col-0 plants, but not in etr1-1 mutants. Thus, the capacity of plants for ethylene perception contributes to the control of root length and its sensitivity to IAA. Roots of etr1-1 were less heavy than in Col-0, indicating that ethylene maintains root mass accumulation. Treatment with IAA caused a decrease in root mass of both genotypes (resulting in a 25 and 10 % decline in the root mass of Col-0 and etr1-1 as compared to the corresponding control), suggesting that IAA may influence root biomass accumulation independently of ethylene. However, sensitivity to ethylene increases plant responsiveness to IAA. Mutation in the auxin receptor decreased the sensitivity of roots to inactivation of ethylene receptors: treatment with 1-MCP resulted in a 40 % decline in the root mass of Col-0 and only a 10 % decrease in tir1. The decrement in sensitivity to auxins in the tir1 mutant decreased the responsiveness of root biomass to 1-MCP-treatment. These data suggest an additive action of ethylene and auxins on accumulation of root biomass.

Keywords:

Arabidopsis thaliana, etr1-1, tir1, ethylene, IAA, root growth, 1-MCP

Downloads

Download data is not yet available.

References

Alarcon, M. V., Lloret, P. G., Salguero, J. 2013. Auxin–ethylene interaction in transversal and longitudinal growth in maize primary root. Botany 91:680–685. https://doi.org/10.1139/cjb-2013-0133

Arkhipova, T. N., Prinsen, E., Veselov, S. Yu., Martinenko, E. V., Melentiev, A. I., Kudoyarova, G. R. 2007. Cytokinin producing bacteria enhances plant growth in drying soil. Plant and Soil 292:305–315. https://doi.org/10.1007/s11104-007-9233-5

Contreras-Cornejo, H. A., López-Bucio, J. S., Méndez-Bravo, A., Macías-Rodríguez, L., Ramos-Vega, M., Guevara-García, Á. A., López-Bucio, J. 2015. Mitogen-activated protein kinase 6 and ethylene and auxin signaling pathways are involved in Arabidopsis root-system architecture alterations by Trichoderma atroviride. Molecular Plant-Microbe Interactions 28(6):701–710. https://doi.org/10.1094/MPMI-01-15-0005-R

Eliasson, L., Bertell, G., Bolander, E. 1989. Inhibitory action of auxin on root elongation not mediated by ethylene. Plant Physiology 91:310–314. https://doi.org/10.1104/pp.91.1.310

Guzman, P., Ecker, J. R. 1990. Exploiting the triple response of Arabidopsis to identity ethylene-related mutants. The Plant Cell 2:513–523. https://doi.org/10.1105/tpc.2.6.513

Ivanov, I. I. 2009. Еndogenous auxins and branching of wheat roots gaining nutrients from isolated compartments. Russian Journal of Plant Physiology 56(2):219–223. https://doi.org/10.1134/S1021443709020101

Korobova, A. V., Vysotskaya, L. B., Vasinskaya, A. N., Kuluev, B. R., Veselov, S. Yu., Kudoyarova, G. R. 2016. Dependence of root biomass accumulation on the content and metabolism of cytokinins in ethylene-insensitive plants. Russian Journal of Plant Physiology 63(5):597–603. https://doi.org/10.7868/S0015330316050079

Kudoyarova, G. R., Dodd, I. C., Veselov, D. S., Rothwell, S. A., Veselov, S. Y. 2015. Common and specific responses to availability of mineral nutrients and water. Journal of Experimental Botany 66:2133–2144. https://doi.org/10.1093/jxb/erv017

Marchant, A., Kargul, J., May, S. T., Muller, P., Delbarre, A., Perrot-Rechenmann, C., Bennett, M. J. 1999. AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin uptake within root apical tissues. The EMBO Journal 18:2066–2073. https://doi.org/10.1093/emboj/18.8.2066

Muday, G. K., Rahman, A., Binder, B. M., Muday, G. K., Rahman, A., Binder, B. M. 2012. Auxin and ethylene: collaborators or competitors? Trends in Plant Science 17:181–195. https://doi.org/10.1016/j.tplants.2012.02.001

Negi, S., Ivanchenko, M. G., Muday, G. K. 2008. Ethylene regulates lateral root formation and auxin transport in Arabidopsis thaliana. The Plant Journal 55:175–187. https://doi.org/10.1111/j.1365-313X.2008.03495.x

Ruzicka, K., Ljung, K., Vanneste, S., Podhorska, R., Beeckman, T., Friml, J., Benkova, E. 2007. Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution. The Plant Cell 19:2197–2212. https://doi.org/10.1105/tpc.107.052126

Shtratnikova, V. Yu., Kudryakova, N. V., Kudoyarova, G. R., Korobova, A. V., Akhiyarova, G. R., Danilova, M. N., Kusnetsov, V. V., and Kulaeva, O. N. 2015. Effects of nitrate and ammonium on growth of Arabidopsis thaliana plants transformed with the ARR5::GUS construct and a role for cytokinins in suppression of disturbances induced by the presence of ammonium. Russian Journal of Plant Physiology 62:741–752. https://doi.org/10.7868/S0015330315060159

Sisler, E. C., Serek, M. 1997. Inhibitors of ethylene responses in plants at the receptor level: recent developments. Physiologia Plantarum 100:577–582. https://doi.org/10.1111/j.1399-3054.1997.tb03063.x

Stepanova, A. N., Yun, J., Likhacheva, A. V., Alonso, J. M. 2007. Multilevel interactions between ethylene and auxin in Arabidopsis roots. The Plant Cell 19:2169–2185. https://doi.org/10.1105/tpc.107.052068

Takahashi, H. 2013. Auxin biology in roots. Plant Root 7:49–64. https://doi.org/10.3117/plantroot.7.49

Tholen, D., Voesenek, L. A. C. J., Poorter, H. 2004. Ethylene insensitivity does not increase leaf area or relative growth rate in Arabidopsis, Nicotiana tabacum, and Petunia x hybrid. Plant Physiology 134:1803–1812. https://doi.org/10.1104/pp.103.034389

Trapeznikov, V. K., Ivanov, I. I., Kudoyarova, G. R. 2003. Еffect of heterogeneous distribution of nutrients on root growth, АБА content and drought resistance of wheat plants. Plant and Soil 252:207–214. https://doi.org/10.1023/A:1024734310214

Vysotskaya, L., Wilkinson, S., Davies, W., Arkhipova, T., Kudoyarova, G. 2011. The effect of competition from neighbours on stomatal conductance in lettuce and tomato plants. Plant Cell and Environment 34:729–737. https://doi.org/10.1111/j.1365-3040.2011.02277.x

Woeste, K. E., Vogel, J. P., Kieber, J. J. 1999. Factors regulating ethylene biosynthesis in etiolated Arabidopsis thaliana seedlings. Physiologia Plantarum 105:478–484. https://doi.org/10.1034/j.1399-3054.1999.105312.x