Dynamics of autotrophic <em>Chlamydomonas reinhardtii</em> metabolome during exponentional and stationary phase

Roman Puzanskiy; Alexey Shavarda; Maria Shishova

Authors

Roman Puzanskiy Saint Petersburg State University, 7–9, Universitetskaya nab., Saint Petersburg, 199034, Russian Federation
Alexey Shavarda Saint Petersburg State University, 7–9, Universitetskaya nab., Saint Petersburg, 199034, Russian Federation
Maria Shishova Saint Petersburg State University, 7–9, Universitetskaya nab., Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0003-3657-2986

Abstract

The growth of autotrophic culture of Chlamydomonas reinhardtii cc-124 was analyzed. Cell density shift was measured and regression model was developed on the basis of approximation by logistic function. For metabolomic analysis of chlamydomonas cells samples were collected at the different time points of culture growth and were analyzed with GC-MS techniques. PCA modeling of the obtained data revealed a correlation of the metabolome and the time of culturing. OPLS (point-by-point pair-wise) modeling of datasets was performed to estimate the links between the growth stage and chlamydomonas metabolome. It was found that the dispersion of metabolite content in cells was strongly related to the time point of cell culture development. SUS-plots visualization of developed models demonstrated strong alteration of the metabolome profile during transition between the first part of exponential growth and the second and during the transition to stationary phase. Correlation analysis and mapping reveal that metabolites are interconnected and clustered in three groups. Refs 33. Figs 5. Tables 2.

Keywords:

Chlamydomonas reinhardtii, exponential growth, metabolomics, OPLS, PCA, stationary phase

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