Heterogeneity of the barrier properties of the colon in rat

  • Viktoria Bekusova Department of General Physiology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0003-3383-053X
  • Ilyas Fatyykhov Department of General Physiology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0003-3607-2447
  • Salah Amasheh Institute of Veterinary Physiology, Freie Universität Berlin, Oertzenweg 19b, Berlin, 14163, Germany https://orcid.org/0000-0002-6365-1949
  • Alexander Markov Department of General Physiology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-2867-044X

Abstract

The incidence of colorectal cancer in different parts of the large intestine is not the same, as tumors more often appear in the distal part of the colon compared to the proximal one. The purpose of this study was to investigate heterogeneity of the barrier properties of the colon and clarify the effects of Prostaglandin E2 and interleukin-1beta on its different parts. An in-depth analysis of short circuit current, transepithelial electrical resistance and paracellular permeability for sodium fluorescein in Ussing chambers showed that the proximal part of the colon was less permeable compared to the distal one and the substances had different effects on the parameters of permeability in different parts of the colon. We suppose that heterogeneity of the barrier properties of the colon and various effects of their regulation by local molecular agents may determine different incidence of pathologies in the colon.

Keywords:

rat, colon, heterogeneity, prostaglandin E2, interleukin-1beta, Ussing chamber, short circuit current, transepithelial electrical resistance, intestinal permeability, barrier properties

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Published
2021-06-30
How to Cite
Bekusova, V., Fatyykhov, I., Amasheh, S., & Markov, A. (2021). Heterogeneity of the barrier properties of the colon in rat. Biological Communications, 66(2), 160–170. https://doi.org/10.21638/spbu03.2021.207
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