Proteins of penial mamilliform glands in closely related <em>Littorina</em> species (Mollusca, Caenogastropoda): variability and possible contribution to reproductive isolation

Arseniy Lobov; Irina Babkina; Arina Maltseva; Natalia Mikhailova; Andrey Granovitch

doi:10.21638/spbu03.2020.206

Authors

Arseniy Lobov Department of Invertebrate Zoology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation; Laboratory of Regenerative Biomedicine, Institute of Cytology, Russian Academy of Sciences, Tikhoretskiy pr., 4, Saint Petersburg, 194064, Russian Federation https://orcid.org/0000-0002-0930-1171
Irina Babkina Department of Invertebrate Zoology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-0406-7021
Arina Maltseva Department of Invertebrate Zoology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0003-1973-4728
Natalia Mikhailova Cell Technologies Center, Institute of Cytology, Russian Academy of Sciences, Tikhoretskiy pr., 4, Saint Petersburg, 194064, Russian Federation https://orcid.org/0000-0003-1650-9330
Andrey Granovitch Department of Invertebrate Zoology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-5203-104X

DOI:

https://doi.org/10.21638/spbu03.2020.206

Abstract

The forces driving reproductive isolation emergence during the process of sympatric speciation are still intensely debated. Mechanisms of gametic isolation (which are known to form rapidly in several models) take the central place in these debates. Nevertheless, the approximative capacity of a few investigated models to other taxa could be questioned, generating demand for the adoption of additional model organisms to study sympatric speciation. The group of closely related species of the genus Littorina (subgenus Neritrema) sympatrically inhabiting seashores are promising. In this study, we performed comparative proteomic analysis of penial tissues of four Neritrema species to identify potential effectors contributing to gametic isolation. Among 272 analyzed proteins, 13 mamilliform gland-specific proteins (possibly transferred to the female during copulation) were detected, as well as five proteins specifically expressed in the epithelium of the penial basal part. Eight of these proteins were species-specific and may be involved in the maintenance of reproductive barriers.

Keywords:

Littorina, proteomics, gamete recognition proteins, mamilliform gland, reproductive isolation, gametic isolation, Mollusca, 2D-DIGE

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