A 3-D-printable plastic trough for serial semithin sectioning
DOI:
https://doi.org/10.21638/spbu03.2018.405Abstract
A simple lightweight 3-D-printable trough to make ribbons of semithin sections is described. The trough is large enough to fit the standard glass slide. The sectioning process is identical to a diamond histo jumbo knife; therefore, the trough may be recommended for specimens which contain hard particles that could damage the knife. The trough is designed to fit the three most common glass knife sizes and can be used with different ultratome models.
Keywords:
3-D reconstruction, 3-D printing, microscopy, semithin sectioning, glass knife, trough
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Articles of Biological Communications are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.