Thioflavin S binds non-amyloid protein structures in lampbrush chromosomes of Gallus gallus domesticus

  • Vera Siniukova Vavilov Institute of General Genetics, Russian Academy of Sciences, Saint Petersburg Branch, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-5505-4693
  • Svetlana Galkina Department of Genetics and Biotechnology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-7034-2466
  • Alexey Galkin Vavilov Institute of General Genetics, Russian Academy of Sciences, Saint Petersburg Branch, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation; Department of Genetics and Biotechnology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-7362-8857

Abstract

Proteins that normally function in amyloid form are found in bacteria, yeast, plants and vertebrates, including humans. In particular, amyloid fibrils and amyloid-like structures are described in the germ cells of various organisms. Recently we showed that in chicken oocytes there are some nuclear structures that are stained by the amyloid-specific dye thioflavin S. Here we demonstrate that thioflavin S binds giant terminal RNP aggregates in chicken lampbrush chromosomes. However, these structures are not stained with Congo red and conformation-dependent anti-amyloid antibodies. Thus, thioflavin S stains chromosome-associated proteins that do not have amyloid properties. These data indicate that thioflavin S must be used with caution when identifying new functional and pathological amyloids.

Keywords:

amyloid, thioflavin S, Congo red, anti-amyloid antibodies, chicken, lampbrush chromosomes

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References

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Published
2022-05-04
How to Cite
Siniukova, V., Galkina, S., & Galkin, A. (2022). Thioflavin S binds non-amyloid protein structures in lampbrush chromosomes of <em>Gallus gallus domesticus</em&gt;. Biological Communications, 67(1), 57–62. https://doi.org/10.21638/spbu03.2022.106
Section
Brief communications