Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes

  • Alsu Saifitdinova Chromas Research Resource Center, Saint Petersburg State University, Oranienbaumskoye sh. 2, 198504, Saint Petersburg, Russian Federation https://orcid.org/0000-0002-1221-479X
  • Svetlana Galkina Department of Genetics and Biotechnology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-7034-2466
  • Valeriia Volodkina Department of Cytology and Histology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation
  • Elena Gaginskaya Department of Cytology and Histology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0003-4967-9053
DOI: https://doi.org/10.21638/11701/spbu03.2017.302

Abstract

Lampbrush chromosomes represent transcriptionally active chromosomes that exist during the extended diplotene phase in the growing ovarian eggs of many vertebrate animals, except therian mammals. They are excellent for studying chromosome structure and aspects of RNA transcription. Because of their giant size they allow a great increase in the resolution for physical gene mapping. Lampbrush chromosomes have no connection with the nuclear envelope, and can be manually dissected from living oocyte nuclei. Here we present the complete protocol for lampbrush chromosome microsurgical isolation from avian and reptilian germinal vesicles. These chromosome preparations can be further used for fluorescence in situ hybridization (FISH) and immunostaining.

Keywords:

nucleus, germinal vesicle, lampbrush chromosomes, meiotic chromosomes, microsurgical isolation, Aves, Reptilia

Downloads

Download data is not yet available.
 

References

Ahmad, M. S. 1970. Development, structure and composition of lampbrush chromosomes in domestic fowl. Canadian Journal of Genetics and Cytology 12: 728–737. https://doi.org/10.1139/g70-095

Andreozzi, L., Federico, C., Motta, S., Saccone, S., Sazanova, A. L., Sazanova, A. A., Smirnov, A. F., Galkina, S. A., Lukina, N. A., and Rodionov, A. V. 2001. Compositional mapping of chicken chromosomes and identification of the gene-richest regions. Chromosome Research 9: 521–532. https://doi.org/10.1023/A:1012436900788

Bellott, D. W., Skaletsky, H., Cho, T.-J., Brown, L., Locke, D., Chen, N., Page, D. C. 2017. Avian W and mammalian Y chromosomes convergently retained dosage-sensitive regulators. Nature Genetics 49: 387–394. https://doi.org/10.1038/ng.3778

Callan, H. G. 1986 Lampbrush Chromosomes. Berlin, Heidelberg: Springer-Verlag.

Callan, H. G., and Lloyd, L. 1960. Lampbrush chromosomes of crested newts Triturus cristatus (Laurenti). Philosophical Transactions of the Royal Society of London, Series B 243: 135–219. https://doi.org/10.1098/rstb.1960.0007

Diaz, M. O. and Gall, J. G. 1985. Giant readthrough transcription units at the histone loci on lampbrush chromosomes of the newt Notophthalmus. Chromosoma 92: 243–253. https://doi.org/10.1007/BF00329807

Gaginskaya, E. R. and Gruzova, M. N. 1969. Peculiarities of the oogenesis in chaffinch. Tsitologiia 11: 1241–1251.

Galkina, S., Derjusheva, S., Fillon, V., Vignal, A., Crooijmans, R., Groenen, M., Rodionov, A. and Gaginskaya, E. 2006. FISH on avian lampbrush chromosomes produces higher resolution gene mapping. Genetica 128: 241–251. https://doi.org/10.1007/s10709-005-5776-7

Gall, J. G. 1954. Lampbrush chromosomes from oocyte nuclei of the newt. Journal of Morphology 94: 283–352. https://doi.org/10.1002/jmor.1050940203

Hutchison, N. 1987. Lampbrush chromosomes of the chicken, Gallus domesticus. Journal of Cell Biology 105: 1493–1500. https://doi.org/10.1083/jcb.105.4.1493

Koecke, H. U., and Muller, M. 1965. Formwechel und Anzahl der Chromosomen bei Huhn und Ente. Naturwissenshchaften 52: 483. https://doi.org/10.1007/BF00626256

Kropotova E. V., and Gaginskaya E. R. 1984. Hromosomy typa lampovyh schetok iz oocytov japonskogo perepela. Dannye svetovoi i elektronnoi mikroskopii [Lampbrush chromosomes from the Japanese quail oocytes. Optical light and electron microscopy data]. Tsitologia 26: 1008–1015.

Macgregor, H. C. 2012. Chromomeres revisited. Chromosome Research 20: 911–924. https://doi.org/10.1007/s10577-012-9310-3

Macgregor, H. C., and Varley, J. M. 1988. Working with animal chromosomes (2nd ed.). Chichester: John Wiley.

Ogawa, A., Solovei, I., Hutchison, N., Saitoh, Y., Ikeda, J-E., Macgregor, H. and Mizuno, S. 1997. Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing Carinatae birds. Chromosome Research 5: 93–101. https://doi.org/10.1023/A:1018461906913

Rückert, J. 1892. Zur Entwicklungsgeschichte des Ovarialeies bei Selachiern. Anatomischer Anzeiger 7: 107–158.

Solinhac, R., Leroux, S., Galkina S, Chazara, O., Feve, K., Vignoles, F., Morisson, M., Derjusheva, S., Bed’hom, B., Vignal, A., Fillon, V. and Pitel, F. 2010. Integrative mapping analysis of chicken microchromosome 16 organization. BMC Genomics 11: 616. https://doi.org/10.1186/1471-2164-11-616

Solovei, I., Gaginskaya, E., Allen, T., and Macgregor, H. 1992. A novel structure associated with a lampbrush chromosome in the chicken, Gallus domesticus. Journal of Cell Science 101: 759–772.

Solovei, I., Gaginskaya, E., Hutchinson, N., and Macgregor, H. 1993. Avian sex chromosomes in the lampbrush form: the ZW lampbrush bivalent from six species of bird. Chromosome Research 1: 153–166. https://doi.org/10.1007/BF00710769

Solovei, I., Gaginskaya, E. R., and Macgregor, H. C. 1994. The arrangement and transcription of telomere DNA sequences at the ends of lampbrush chromosomes of birds. Chromosome Research 2: 460–470. https://doi.org/10.1007/BF01552869

Solovei, I., Macgregor, H., and Gaginskaya, E. 1995. Single stranded nucleic acid binding structures on chicken lampbrush chromosomes. Journal of Cell Science 108: 1391–1396.

Zlotina, A., Galkina, S., Krasikova, A., Crooijmans, R. P. M. A., Groenen, M. A. M., Gaginskaya, E. and Derjusheva, S. 2010. Precise centromere positioning on chicken chromosome 3. Cytogenetics and Genome Research 129: 310–313. https://doi.org/10.1159/000314923
Biological Communications
Published
2017-12-29
How to Cite
Saifitdinova, A., Galkina, S., Volodkina, V., & Gaginskaya, E. (2017). Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes. Biological Communications, 62(3), 165–168. https://doi.org/10.21638/11701/spbu03.2017.302
Issue
Vol. 62 No. 3 (2017)
Section
Jubilee section

Articles of Biological Communications are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.