Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes
DOI:
https://doi.org/10.21638/11701/spbu03.2017.302Abstract
Lampbrush chromosomes represent transcriptionally active chromosomes that exist during the extended diplotene phase in the growing ovarian eggs of many vertebrate animals, except therian mammals. They are excellent for studying chromosome structure and aspects of RNA transcription. Because of their giant size they allow a great increase in the resolution for physical gene mapping. Lampbrush chromosomes have no connection with the nuclear envelope, and can be manually dissected from living oocyte nuclei. Here we present the complete protocol for lampbrush chromosome microsurgical isolation from avian and reptilian germinal vesicles. These chromosome preparations can be further used for fluorescence in situ hybridization (FISH) and immunostaining.
Keywords:
nucleus, germinal vesicle, lampbrush chromosomes, meiotic chromosomes, microsurgical isolation, Aves, Reptilia
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Published
2017-12-29
How to Cite
Saifitdinova, A., Galkina, S., Volodkina, V., & Gaginskaya, E. (2017). Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes. Biological Communications, 62(3), 165–168. https://doi.org/10.21638/11701/spbu03.2017.302
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Articles of Biological Communications are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.
