Influence of exogenous antioxidants on the generation of superoxide anion-radicals in the cucumber leaves under low temperature and Cu2+ stress
The effect of exogenous ascorbic acid and glutathione on the oxidative stress in cucumber leaves under chilling temperature and Cu2+ was studied. Ascorbic acid showed a pronounced antioxidant effect only at chilling temperature 3 °С, whereas at 25 °С it has a prooxidant effect that results in increased O2˙– generation. Glutathione provoked reducing of O2˙– level under both temperatures. Cu2+ in concentration 10–3 М induced O2˙– generation in leave cells under both 25 °С and 3 °С, whereas Cu2+ addition in lower concentration 10–5 М at 3 °С resulted in decreased production of O2˙–. The combined action of glutathione and Cu2+ 10–5 М was shown to lead to reduced O2˙– generation, but the effect was opposite when glutathione was given together with Cu2+ in concentration 10–3 М. It is concluded that glutathione is more effective in reducing of O2˙– generation than ascorbic acid but its antioxidant activity appears to be inhibited in the presence of high Cu2+ concentrations. Refs 27. Figs 2. Tables 2.
oxidative stress, low temperature stress, antioxidants, ascorbic acid, glutathione, superoxide anion-radical, Cu2
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