Influence of exogenous antioxidants on the generation of superoxide anion-radicals in the cucumber leaves under low temperature and Cu2+ stress

  • Alexander Lukatkin Mordovia State University, Saransk, Russian Federation
  • Svetlana Eshkina Mordovia State University, Saransk, Russian Federation
  • Natalia Osmolovskaya Department of Plant Physiology and Biochemistry, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0001-8764-8552

Abstract

The effect of exogenous ascorbic acid and glutathione on the oxidative stress in cucumber leaves under chilling temperature and Cu2+ was studied. Ascorbic acid showed a pronounced antioxidant effect only at chilling temperature 3 °С, whereas at 25 °С it has a prooxidant effect that results in increased O2˙ generation. Glutathione provoked reducing of O2˙ level under both temperatures. Cu2+ in concentration 10–3 М induced O2˙ generation in leave cells under both 25 °С and 3 °С, whereas Cu2+ addition in lower concentration 10–5 М at 3 °С resulted in decreased production of O2˙. The combined action of glutathione and Cu2+ 10–5 М was shown to lead to reduced O2˙ generation, but the effect was opposite when glutathione was given together with Cu2+ in concentration 10–3 М. It is concluded that glutathione is more effective in reducing of O2˙ generation than ascorbic acid but its antioxidant activity appears to be inhibited in the presence of high Cu2+ concentrations. Refs 27. Figs 2. Tables 2.

Keywords:

oxidative stress, low temperature stress, antioxidants, ascorbic acid, glutathione, superoxide anion-radical, Cu2

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Published
2013-12-30
How to Cite
Lukatkin, A., Eshkina, S., & Osmolovskaya, N. (2013). Influence of exogenous antioxidants on the generation of superoxide anion-radicals in the cucumber leaves under low temperature and Cu<sup>2+</sup&gt; stress. Biological Communications, (4), 65–73. Retrieved from https://biocomm.spbu.ru/article/view/1326
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