Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic

  • Denis Karlov All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0002-9030-8820
  • Anna Sazanova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0002-4808-320X
  • Irina Kuznetsova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0003-0260-7677
  • Nina Tikhomirova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0001-8530-6698
  • Zhanna Popova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0003-3558-7190
  • Yuriy Osledkin All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0002-4216-3372
  • Nikita Demidov Arctic and Antarctic Research Institute, ul. Beringa, 38, Saint Petersburg, 199397, Russian Federation https://orcid.org/0000-0002-3462-7747
  • Andrey Belimov All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0002-9936-8678
  • Vera Safronova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0003-4510-1772


Twenty-nine strains were isolated from two samples of the permafrost active layer of the Spitsbergen archipelago. The estimated number of bacteria ranged from 4.0⋅104 to 1.7⋅107 CFU∙g-1. As a result of sequencing of the 16S rRNA (rrs) genes, the isolates were assigned to 13 genera belonging to the phyla Actinobacteria, Proteobacteria (classes α, β, and γ), Bacteroidetes, and Firmicutes. Six isolates belonged to the rhizobial genus Mesorhizobium (order Rhizobiales). A plant nodulation assay with seedlings of legume plants Astragalus norvegicus, A. frigidus, A. subpolaris and Oxytropis sordida, originated from Khibiny (Murmansk region, Russia) and inoculated with Mesorhizobium isolates, showed the inability of these strains to form nodules on plant roots. Symbiotic (sym) genes nodC and nodD were not detected in Mesorhizobium strains either.


legume plants, root nodule bacteria, 16S rRNA genes, permafrost soil, Spitsbergen


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How to Cite
Karlov, D., Sazanova, A., Kuznetsova, I., Tikhomirova, N., Popova, Z., Osledkin, Y., Demidov, N., Belimov, A., & Safronova, V. (2021). Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic. Biological Communications, 66(1), 73–82. https://doi.org/10.21638/spbu03.2021.109
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