Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic

Denis Karlov; Anna Sazanova; Irina Kuznetsova; Nina Tikhomirova; Zhanna Popova; Yuriy Osledkin; Nikita Demidov; Andrey Belimov; Vera Safronova

doi:10.21638/spbu03.2021.109

Authors

Denis Karlov All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0002-9030-8820
Anna Sazanova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0002-4808-320X
Irina Kuznetsova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0003-0260-7677
Nina Tikhomirova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0001-8530-6698
Zhanna Popova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0003-3558-7190
Yuriy Osledkin All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0002-4216-3372
Nikita Demidov Arctic and Antarctic Research Institute, ul. Beringa, 38, Saint Petersburg, 199397, Russian Federation https://orcid.org/0000-0002-3462-7747
Andrey Belimov All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0002-9936-8678
Vera Safronova All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel'skogo, 3, Saint Petersburg, 190608, Russian Federation https://orcid.org/0000-0003-4510-1772

DOI:

https://doi.org/10.21638/spbu03.2021.109

Abstract

Twenty-nine strains were isolated from two samples of the permafrost active layer of the Spitsbergen archipelago. The estimated number of bacteria ranged from 4.0⋅10⁴ to 1.7⋅10⁷ CFU∙g^-1. As a result of sequencing of the 16S rRNA (rrs) genes, the isolates were assigned to 13 genera belonging to the phyla Actinobacteria, Proteobacteria (classes α, β, and γ), Bacteroidetes, and Firmicutes. Six isolates belonged to the rhizobial genus Mesorhizobium (order Rhizobiales). A plant nodulation assay with seedlings of legume plants Astragalus norvegicus, A. frigidus, A. subpolaris and Oxytropis sordida, originated from Khibiny (Murmansk region, Russia) and inoculated with Mesorhizobium isolates, showed the inability of these strains to form nodules on plant roots. Symbiotic (sym) genes nodC and nodD were not detected in Mesorhizobium strains either.

Keywords:

legume plants, root nodule bacteria, 16S rRNA genes, permafrost soil, Spitsbergen

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References

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