Spontaneous activity in neuromuscular synapse of <em>Drosophila melanogaster</em> larvae with human <em>APP</em> gene expression

Ekaterina Saburova; Alexander Vasiliev; Violetta Kravtsova; Olga Bolshakova; Svetlana Sarantseva; Igor Krivoi

Authors

Ekaterina Saburova Saint Petersburg State University, 7–9, Universitetskaya nab., Saint Petersburg, 199034, Russian Federation
Alexander Vasiliev Saint Petersburg State University, 7–9, Universitetskaya nab., Saint Petersburg, 199034, Russian Federation
Violetta Kravtsova Saint Petersburg State University, 7–9, Universitetskaya nab., Saint Petersburg, 199034, Russian Federation
Olga Bolshakova B. P. Konstantinov St. Petersburg Nuclear Physics Institute, National Research Centre «Kurchatov Institute», Orlova Roscha, Gatchina, Leningrad district, 188300, Russian Federation
Svetlana Sarantseva B. P. Konstantinov St. Petersburg Nuclear Physics Institute, National Research Centre «Kurchatov Institute», Orlova Roscha, Gatchina, Leningrad district, 188300, Russian Federation
Igor Krivoi Saint Petersburg State University, 7–9, Universitetskaya nab., Saint Petersburg, 199034, Russian Federation

Abstract

According to amyloid cascade hypothesis the main role in pathogenesis of Alzheimer’s disease plays β-amyloid peptide, which is the product of proteolytic processing of amyloid precursor protein (APP). In regards to APP, physiological role of this protein itself remain incompletely understood. A convenient model for such experiments is Drosophila melanogaster, which does not contain APP and β-secretase genes. So in transgenic lines of Drosophila melanogaster it’s possible to study effects of APP and β-amyloid independently. In our experiments on neuromuscular synapse of Drosophila melanogaster larvae with human APP gene expression a decrease of spontaneous quantal transmitter release frequency without alteration of its random nature was demonstrated. Frequency of spontaneous transmitter release was not affected at decreased level of APP and production of β-amyloid in conditions of co-expression of human APP and β-secretase genes. These changes give evidences for alteration of synaptic vesicle exocytosis mechanism which is specific for APP.

Keywords:

neuromuscular synapse, Drosophila melanogaster, APP, quantal transmitter release

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