Homoarginine and ornithine production during C2C12 myogenic differentiation

Authors

  • Alexandr Zhloba Biochemical Department of Scientific Centre, Pavlov First Saint Petersburg State Medical University, L'va Tolstogo Str., 6–8, Saint Petersburg, 197022, Russian Federation https://orcid.org/0000-0003-0605-7617
  • Tatiana Subbotina Biochemical Department of Scientific Centre, Pavlov First Saint Petersburg State Medical University, L'va Tolstogo Str., 6–8, Saint Petersburg, 197022, Russian Federation https://orcid.org/0000-0002-2278-8391
  • Stanislava Prikhodko Institute of Molecular Biology and Genetics, Almazov Federal Medical Research Centre, ul. Akkuratova, 2, Saint Petersburg, 197341, Russian Federation https://orcid.org/0000-0001-7018-6398
  • Alexandr Khudiakov Institute of Molecular Biology and Genetics, Almazov Federal Medical Research Centre, ul. Akkuratova, 2, Saint Petersburg, 197341, Russian Federation
  • Natalia Smolina Institute of Molecular Biology and Genetics, Almazov Federal Medical Research Centre, ul. Akkuratova, 2, Saint Petersburg, 197341, Russian Federation; ITMO University, Saint Petersburg, Russian Federation https://orcid.org/0000-0002-3339-0688
  • Anna Kostareva Institute of Molecular Biology and Genetics, Almazov Federal Medical Research Centre, ul. Akkuratova, 2, Saint Petersburg, 197341, Russian Federation; ITMO University, Saint Petersburg, Russian Federation; Department of Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden https://orcid.org/0000-0002-9349-6257

DOI:

https://doi.org/10.21638/spbu03.2018.303

Abstract

Assessment of cellular rates of amino acid consumption and release in vitro allows the study of cell culture in a time-course experiment without any cell damage. Determination of the release of amino acid metabolites that initially were not present in the media provides more reliable information about the processes of growth and differentiation in comparison with determination of amino acid consumption rates. Homoarginine (hArg), a derivative of arginine, is generated as the minor product in the reaction catalyzed by L-Arginine: glycine amidinotransferase, where L-lysine serves as an acceptor for amidine group instead of glycine. Ornithine is another product generated in this reaction from arginine. Thus, the goal of the present study was to evaluate the rate of hArg and ornithine accumulation in comparison to the rate of consumption of other amino acids in the course of C2C12 myoblast differentiation. The release time profiles were similar for hArg and ornithine, with the maximum corresponding to the second day of differentiation. The shift for hArg at this time point was detected with greater reliability (p < 0.002) than for ornithine and other amino acids. We suggest that hArg and ornithine could serve as markers to monitor the processes of myoblasts growth and differentiation.

Keywords:

amino acids, homoarginine, myogenic differentiation, ornithine

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References

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Biological Communications

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Published

2018-11-30

How to Cite

Zhloba, A., Subbotina, T., Prikhodko, S., Khudiakov, A., Smolina, N., & Kostareva, A. (2018). Homoarginine and ornithine production during C2C12 myogenic differentiation. Biological Communications, 63(3), 174–179. https://doi.org/10.21638/spbu03.2018.303

Issue

Vol. 63 No. 3 (2018)

Section

Full communications

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Articles of Biological Communications are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.

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