Rate of spontaneous polyploidization in haploid yeast Saccharomyces cerevisiae

  • Yulia Andreychuk Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina, 3, Moscow, 117971, Russian Federation https://orcid.org/0000-0003-0957-9840
  • Anna Zhuk ITMO University, Kronverkskiy pr., 49, Saint Petersburg, 197101, Russian Federation https://orcid.org/0000-0001-8683-9533
  • Elena Tarakhovskaya Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina, 3, Moscow, 117971, Russian Federation; Department of Plant Physiology and Biochemistry, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-5341-2813
  • Sergei Inge-Vechtomov Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina, 3, Moscow, 117971, Russian Federation; Department of Genetics and Biotechnology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-2832-6825
  • Elena Stepchenkova Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina, 3, Moscow, 117971, Russian Federation; Department of Genetics and Biotechnology, Faculty of Biology, Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, 199034, Russian Federation https://orcid.org/0000-0002-5854-8701

Abstract

Polyploidization is involved in a variety of biological processes. It is one of the principal mechanisms of new species formation since it provides material for gene diversification and subsequent selection. Multiple cases of polyploidization were registered in different branches of the evolutionary tree of eukaryotes. Besides its role in evolution, polyploidization affects gene expression in living cells: pathological genome duplications often happen in cancer cells. The mechanisms and consequences of polyploidization are being studied extensively. However, quantitative determination of the polyploidization rate is challenging due to its low frequency and the absence of selective genetic markers that would phenotypically distinguish between haploids and polyploids. Our work describes a robust and straightforward method for discriminating haploid and polyploid states in the yeast Saccharomyces cerevisiae, a model organism for studying different aspects of polyploidization. The measurement of polyploidization rate showed that in yeast cells this process is mainly caused by autodiploidization rather than mating-type switching followed by hybridization.

Keywords:

Saccharomyces cerevisiae, polyploidization, haploid, diploid, whole-genome duplication

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References

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Published
2022-06-24
How to Cite
Andreychuk, Y., Zhuk, A., Tarakhovskaya, E., Inge-Vechtomov, S., & Stepchenkova, E. (2022). Rate of spontaneous polyploidization in haploid yeast <em>Saccharomyces cerevisiae</em&gt;. Biological Communications, 67(2), 88–96. https://doi.org/10.21638/spbu03.2022.202
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