Diet affects body color and energy metabolism in the Baikal endemic amphipod <em>Eulimnogammarus cyaneus</em> maintained in laboratory conditions

Alexandra Saranchina; Polina Drozdova; Andrei Mutin; Maxim Timofeyev

doi:10.21638/spbu03.2021.306

Authors

Alexandra Saranchina Irkutsk State University, ul. Karla Marksa, 1, Irkutsk, 664003, Russian Federation https://orcid.org/0000-0002-5756-1525
Polina Drozdova Irkutsk State University, ul. Karla Marksa, 1, Irkutsk, 664003, Russian Federation; Baikal Research Centre, ul. Lenina, 21, Irkutsk, 664003, Russian Federation https://orcid.org/0000-0003-3955-6105
Andrei Mutin Irkutsk State University, ul. Karla Marksa, 1, Irkutsk, 664003, Russian Federation https://orcid.org/0000-0002-3092-5376
Maxim Timofeyev Irkutsk State University, ul. Karla Marksa, 1, Irkutsk, 664003, Russian Federation; Baikal Research Centre, ul. Lenina, 21, Irkutsk, 664003, Russian Federation https://orcid.org/0000-0002-5250-6818

DOI:

https://doi.org/10.21638/spbu03.2021.306

Abstract

Proper diet is critical for laboratory-reared animals, as it may affect not only their welfare, but also experimental results. Amphipods (Crustacea: Amphipoda) play important roles in ecosystems and are often used in environmental research. Endemic amphipods from the ancient Lake Baikal are promising for laboratory bioassays; however, there are currently no laboratory cultures. In this work, we determine how different diets affect the color and metabolism of a laboratory-reared Baikal amphipod, Eulimnogammarus cyaneus. We found that in freshly collected blue-colored animals, body color correlated with total carotenoid content. Total carotenoid levels did not differ after long-term (two months) feeding with a close to natural carotenoid-enriched, or even a carotenoid-depleted diet. Nevertheless, antennae color was closer to red in the natural-like diet group. It is likely that the carotenoids from the commercial diet are not properly metabolized in E. cyaneus. The animals fed commercial diets had a higher glycogen content, which may signify a higher metabolic rate. Overall, we show that a carotenoid-enriched diet optimized for decapods is not optimal for amphipods, likely due to different carotenoid compositions, and the diet for long-term rearing of E. cyaneus and other Baikal amphipods requires supplementation.

Keywords:

carotenoids, diet, laboratory rearing, Baikal, Amphipoda, Crustacea, Decapoda, culture, metabolites

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References

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