Comparative study of the fullness of dwarf Siberian pine seeds <em>Pinus pumila</em> (Pall.) Regel from places of natural growth and collected from plants introduced in northwestern Russia by microfocus X-ray radiography to predict their sowing qualities

Anastasia Karamysheva; Lev Trofimuk; Nikolay Priyatkin; Mikhail Arkhipov; Ludmila Gusakova; Polina Sshukina; Nikolay Staroverov; Nikolay Potrakhov

doi:10.21638/spbu03.2020.403

Authors

Anastasia Karamysheva Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova, 2, Saint Petersburg, 197376, Russian Federation https://orcid.org/0000-0003-1109-5810
Lev Trofimuk Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova, 2, Saint Petersburg, 197376, Russian Federation; Scientific Experimental Station of the Komarov Botanical Institute, Plodovoe Village, Priozersk District, Leningrad Region, Russian Federation https://orcid.org/0000-0002-1007-5087
Nikolay Priyatkin Plant Biophysics Division, Agrophysical Research Institute, Grazhdanskiy pr., 14, Saint Petersburg, 195220, Russian Federation https://orcid.org/0000-0002-5974-4288
Mikhail Arkhipov Plant Biophysics Division, Agrophysical Research Institute, Grazhdanskiy pr., 14, Saint Petersburg, 195220, Russian Federation; Crop Science Division, Northwestern Center of Interdisciplinary Research of Problems of Food Maintenance, Shosse Podbel'skogo, 7, Saint Petersburg, 196608, Russian Federation https://orcid.org/0000-0002-6903-6971
Ludmila Gusakova Plant Biophysics Division, Agrophysical Research Institute, Grazhdanskiy pr., 14, Saint Petersburg, 195220, Russian Federation https://orcid.org/0000-0002-6178-2953
Polina Sshukina Plant Biophysics Division, Agrophysical Research Institute, Grazhdanskiy pr., 14, Saint Petersburg, 195220, Russian Federation; Crop Science Division, Northwestern Center of Interdisciplinary Research of Problems of Food Maintenance, Shosse Podbel'skogo, 7, Saint Petersburg, 196608, Russian Federation https://orcid.org/0000-0002-5223-8374
Nikolay Staroverov Department of Electronic Systems and Devices, The First Electrotechnical University “LETI”, ul. Professora Popova, 5, Saint Petersburg, 197376, Russian Federation https://orcid.org/0000-0002-4404-5222
Nikolay Potrakhov Department of Electronic Systems and Devices, The First Electrotechnical University “LETI”, ul. Professora Popova, 5, Saint Petersburg, 197376, Russian Federation https://orcid.org/0000-0001-8806-0603

DOI:

https://doi.org/10.21638/spbu03.2020.403

Abstract

As a result of analysis of the quality of Pinus pumila seeds by the method of microfocus X-ray radiography in combination with the automatic analysis of digital X-ray images, it was found that the best characteristics of individual structures and organs of seeds were demonstrated in samples collected from trees growing at site 71 of BIN RAS; the worst — from seeds taken from South Sakhalin. The seeding qualities of Pinus pumila seed samples were determined by standard methods. Based on analysis of the characteristics of digital X-ray images of Pinus pumila seeds, it was found that a seed sample from site 71 BIN RAS was characterized by the high level of embryo area 4.19±0.49 mm², maximum embryo to thalus ratio 60.95±7.45 %, high level of endosperm area 23.93±1.24 mm², and maximum ratio square of embryo area 9.45 ±1.17 %. The same sample was characterized by a maximum weight of 1000 seeds and a maximum absolute and soil germination ratio, compared to other samples. The obtained data showed that Pinus pumila seeds collected from plants introduced in northwestern Russia by most parameters are not inferior or are even superior to seeds from the natural range.

Keywords:

Dwarf Siberian pine, Pinus pumila, seed quality, X-ray radiography of seeds, seed image analysis, soil germination of seeds

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