ITS1-gene 5.8S rRNA-ITS2 and trnL-trnF sequence variability during the divergence of Elymus L. species of the flora of Siberia and Russian Far East

Authors

  • Kseniya Dobryakova V. L. Komarov Botanical Institute of the RAS, 2, ul. Professora Popova, St. Petersburg, 197376, Russian Federation
  • Nikolay Nosov V. L. Komarov Botanical Institute of the RAS, 2, ul. Professora Popova, St. Petersburg, 197376, Russian Federation

DOI:

https://doi.org/10.21638/spbu03.2015.401

Abstract

The phylogenetic relationship of Elymus species were analyzed by molecular phylogenetic methods. Comparative analysis of the sequences ITS1-5.8S rRNA gene-ITS2 of the nuclear genome species of genus Elymus and kinship group showed that the species of the genus Elymus have 3 haplotypes. Нaplotype A was found in Elymus species (sections: Turczaninovia, Goulardia и Elymus) and Elytrigia geniculata; haplotype B was found in Elymus species (sections: Turczaninovia, Goulardia); haplotype C (sections: Goulardia и Clinelymopsis) and E. repens. Comparative analysis of the sequences trnL-trnF of the chloroplast genome of genus Elymus related species showed that the species of the genus Elymus form strongly supported clade. The phylogenetic trees were constructed using Bayesian method. Refs 22. Figs 2. Tables 3.

Keywords:

Elymus, molecular phylogeny, hybridization

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References

Tsvelev N. N. O rode Elymus L. (Poaceae) v Rossii [On the genus Elymus L. (Poaceae) in Russia]. Bot. zhurn. [Bot. Zhurn.], 2008, vol. 93, no. 10, pp. 1587–1596. (In Russian)

Dizkirici A., Kaya Z., Cabi E., Dogan M. Phylogenetic relationships of Elymus L. and related genera (Poaceae: Triticeae Dumort.) based on the nuclear ribosomal internal transcribed spacer sequences. Turk. J. of Botany, 2010, vol. 3, no. 6, pp. 467–478.

Tsvelev N. N. Zlaki SSSR [Grasses of the USSR]. Leningrad, Nauka Publ., 1976. 788 p. (In Russian)

Mason-Gamer R. J. Phylogeny of a Genomically Diverse Group of Elymus (Poaceae) Allopolyploids Reveals Multiple Levels of Reticulation. PLoS ONE. 2013. 8:e78449.

Liu Q., Ge S., Tang H., Zhang X., Zhu G., Lu B. R. Phylogenetic relationships in Elymus (Poaceae: Triticeae) based on the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences. New Phytol., 2006, vol. 170, no. 2, pp. 411–420.

Matveeva T. V., Pavlova O. A., Bogomaz D. I., Demkovich A. E., Lutova L. A. Molekuliarnye markery dlia vidoidentifikatsii i filogenetiki rastenii [Molecular markers for plant species identifi cation and phylogenetics]. Ekologicheskaia genetika [Ecological genetics], 2011, vol. 9, no. 1, pp. 32–43. (In Russian)

Alvarez E., Wendel J. F. Ribosomal ITS sequences and plant phylogenetic inference. Molecular Phylogenetics and Evolution, 2003, vol. 29, pp. 417–434.

Matsushima R., Yamashita J., Kariyama S., Enomoto T., Sakamoto W. A Phylogenetic revaluation of morphological variations of starch grains among Poaceae species. J. Appl. Glycosci., 2013, vol. 60, pp. 37–44.

Mason-Gamer R. J., Orme N. L., Anderson C. M. Phylogenetic analysis of North American Elymus and the monogenomic Triticeae (Poaceae) using three chloroplast DNA data sets. Genome, 2002, vol. 45, no. 6, pp. 991–1002.

Liu Z., Chen Z., Pan J., Li X., Su M., Wang L., Li H., Liu G. Phylogenetic relationships in Elymus (Poaceae: Triticeae) revealed by the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences. Mol. Phylogenet. Evol., 2008, vol. 46, no. 1, pp. 278–289.

Rabey H. E. Comparison of the internal transcribed spacer region (ITS) of the ribosomal RNA genes in wild and cultivated two and six-rowed barleys (Hordeum vulgare L.). Mol. Biol. Rep., 2014, vol. 41, no. 2, pp. 849–854.

Alonso A., Bull R. D., Acedo C., Gillespie L. J. Design of plant-specific PCR primers for the ETS region with enhanced specificity for tribe Bromeae and their application to other grasses (Poaceae). Botany, 2014, vol. 92, no. 10, pp. 693–699.

Saarela J. M., Peterson P. M., Keane R. M., Cayouette J., Graham S. W. Molecular phylogenetics of the genus Bromus (Poaceae: Pooideae) based on nuclear and chloroplast DNA sequence data. Aliso, 2007, vol. 23, pp. 450–467.

Rodionov A. V., Nosov N. N., Kim E. S., Machs E. M., Punina E. O., Probatova N. S. Proiskhozhdenie poliploidnykh genomov miatlikov (Poa L.) i fenomen potoka genov mezhdu Severnoi Patsifikoi i subantarkticheskimi ostrovami [The origin of polyploid genomes of bluegrasses Poa L. and gene flow between northern pacific and sub-antarctic islands]. Genetika [Russ. J. Genet.], 2010, vol. 46, no. 12, pp. 1598–1608. (In Russian)

Doyle J. J., Doyle J. L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 1987, vol. 19, pp. 11–15.

Ridgway K. P., Duck J. M., Young J. P. W. Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trnL (UAA) intron. BMC Ecology, 2003, vol. 3(8e).

White T. J., Bruns T., Lee S., Taylor J. W. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications. Eds M. A. Innis, D. H. Gelfand, J. J. Sninsky, T. J. White. New York, Academic Press, Inc., 1990, pp. 315–322.

Taberlet P., Gielly L., Pautou G., Bouvet J. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molec. Biology, 1991, vol. 17, pp. 1105–1109.

Rodionov A. V., Tiupa N. B., Kim E. S., Machs E. M., Loskutov I. G. Genomnaia konstitutsiia avtotetraploidnogo ovsa Avena macrostachya, vyiavlennaia putem sravnitel'nogo analiza posledovatel'nostei ITS1 i ITS2: k voprosu ob evoliutsii kariotipov ovsov i ovsiugov na rannikh etapakh divergentsii vidov roda Avena [Genomic configuration of the autotetraploid oat species Avena macrostachya inferred from comparative analysis of ITS1 and ITS2 sequences: on the oat caryotype evolution during the early events of the Avena species divergence]. Genetika [Russ. J. Genet.], 2005, vol. 41, no. 5, pp. 646–656. (In Russian)

Redinbaugh M. G., Jones T. A., Zhang Y. T. Ubiquity of the St chloroplast genome in St containing Triticeae polyploids. Genome, 2000, vol. 43, pp. 846–852.

Kotseruba V., Gernand D., Meister A., Houben A. Uniparental loss of ribosomal DNA in the allotetraploid grass Zingeria trichopoda (2n=8). Genome, 2003, vol. 46, pp. 156–163.

Blattner P. R. Phylogenetic analysis of Hordeum (Poaceae) as inferred by nuclear rDNA ITS sequences. Molec. Phylogenet. and Evolut., 2004, vol. 33, pp. 289–299.

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Published

2015-12-25

How to Cite

Dobryakova, K., & Nosov, N. (2015). ITS1-gene 5.8S rRNA-ITS2 and trnL-trnF sequence variability during the divergence of <em>Elymus</em> L. species of the flora of Siberia and Russian Far East. Biological Communications, (4), 4–17. https://doi.org/10.21638/spbu03.2015.401

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