Variability of the hepatic α-amilase activities in the liver of fish species from the Sevastopol bays, Black Sea

  • Natalya Kuzminova A.O. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, Nakhimov Ave., 2, Sevastopol, 299011, Crimea, Russian Federation


This paper presents the results of a study of α-amylase activity in liver of widely represented species of twelve Black sea fish. Comparative analysis of interspecies differences showed that the medium level of enzyme activity is very high only in liver of annular seabream and horse mackerel. The Black Sea predators (scorpion fish, stargazer, shore rockling, whiting) have low values of the medium level of enzyme activity (range from 0,02 to 62,79 mg / s / g of protein). Enzyme activity did not depend on fish sex, their age and location. Research has established lack of any link between activity of enzyme and age for both the scorpion fish and high body pickarel. While studying the effects of seasonal features on enzyme activity in the liver of two dominant species in the coastal zone, it was concluded that enzyme activity is lower in winter than in other seasons.


fish, digestive enzyme, liver, Black Sea


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Afanasyeva, O. M. 2011. The assessment of zoohygienic safety of monklavitу-1 and its use for calves growing. Author. Dis. Cand. Thesis. Veterinary sanitation, ecology, pet hygiene and veterinary sanitary expertise. Saint Petersburg. 20 p. (In Russian)

Alarcon, F. J., Martíınez, T. F., Díaz, M., and Moyano, F. J. 2001. Characterization of digestive carbohydrase activity in the gilthead seabream (Sparus aurata). Hydrobiologia 445:199–204.

Alyomov, S. V., Guseva, E. V., Solov'yova, O. V., Tihonova, E. A., Burdiyan, N. V., and Doroshenko, Yu. V. 2015. The results of complex sanitary-biological studies of Pesochnaya bay (Sevastopol region). Scientific notes of the Crimean state university V. I. Vernadskogo named. «Biology, Chemistry» 67(2):3–17. (In Russian)

Alvarez-González, C. A., Cervantes-Trujano, M., Tovar-Ramirez, D., Conklin, D. E., Nolasko, H., Gisbert, E., and Piedrahita, R. 2006. Development of digestive enzymes in California halibut Paralichthys californicus larvae. Fish Physiology and Biochemistry 31:83–93.

Al-Tameemi, R., Aldubaikul, A., and Salman, N. A. 2010. Comparative study of α-amylase activity in three Cyprinid species of different feeding habits from Southern Iraq. Turkish Journal of Fisheries and Aquatic Sciences 10:411‒414.

Caraway, W. T. 1959. A stable starch substrate for the determination of amylase in serum and other body fluids. American Journal Clinical Pathologic 32:97‒99.

Farhoudi, A., Abedian Kenari, A. M., Nazari, R. M., and Mackhdoomi, C. H. 2013. Changes of digestive enzymes of activity in common carp (Cyprinus carpio) during larval ontogeny. Iranian journal of Fisheries Sciences 12(2):320–334.

Ferna´ndez, I., Moyano, F. J., D´ıaz, M., and Mart´ınez, T. 2001. Characterization of α-amylase activity in five species of Mediterranean sparid fishes Sparidae, Teleostei. Journal of Experimental Marine Biology and Ecology 262:1–12.

Ferreira, С. M., Field, C. L., and Tuttle, A. D. 2010. Hematological and plasma biochemical parameters of aquarium-maintained cownose rays. Journal of Aquatic Animal Health 22:123–128.

Gisbert, E., Giménez, G., Fernández, I., Kotzamanis, Y., and Estévez, A. 2009. Development of digestive enzymes in common dentex Dentex dentex during early ontogeny. Aquaculture 287:381–387.

Gottlieb Almeida, A. P., Zardo, E. L., Toni, C., Behr, E. R., da Silva, L. P., Vieira, J. P., Loro, V. L., and Baldisserotto, B. 2018. Composition of gastrointestinal content, protease and lipase activities in summer and winter of four freshwater siluriforms (Teleostei: Actinopterygii) with two different feeding habits. Zoologia 35:e13286

Hidalgo, M. C., Urea, E., and Sanz, A. 1999. Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities. Aquaculture 170:267‒283.

Kandyuk, R. P. 1966. Comparative study of the activity and the thermal stability of digestive enzymes of plankton and benthic fish northwestern part of the Black Sea. Author. Diss. Thesis. Odessa. Hydrobiology. 22 p. (In Russian)

Klimova, T. N. 2010. Ichthyoplankton of the coastal waters of south-western Crimea in 2002–2008. Marine ecological journal IX(1):39‒52. (In Russian)

Krogdahl, A., Hemre, G. I., and Mommsen, T. P. 2005. Carbohydrates in fish nutrition: digestion and absorption in postlarval stages. Aquaculture Nutrition 11(2):103‒122.

Kuzmina, V. V. 1977. Features of membrane digestion of freshwater bony fish. Journal of Ichthyology 17(1):111‒119. (In Russian)

Kuz'mina, V. V. 1996. Influence of age on digestive enzyme activity in some freshwater teleosts. Aquaculture 148:25‒37.

Kuz’mina, V. V., Golovanova, I. L., and Izvekova, G. I. 1996. Influence of temperature and season on some characteristics of intestinal mucosa carbohydrases in six freshwater fishes. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 113:255‒260.

Kuzminova, N. S. 2006. Study of the toxic effect of domestic sewage on marine organisms. Diss. Cand. Thesis. Ecology. Moscow. 168 p. (In Russian)

Kuzminova, N. S. 2010. Species and ecological characteristics of lysozyme activity in the serum of some species of Black Sea fish. Ecological systems and devices 2:22‒27. (In Russian)

Kuzminova, N., Dorokhova, I., and Rudneva, I. 2014a. Age-dependent changes of Mediterranean Trachurus mediterraneus male and female from coastal waters of Sevastopol (Black Sea, Ukraine). Turkish Journal of Fisheries and Aquatic Sciences 14:183–192.

Kuzminova, N. S., Kulakovskaya, E. V., and Yakimova, K. V. 2014b. The concentration of circulating immune complexes in the blood of Black Sea fishes in the modern period; pp. 321‒325 in Abstracts of Intern. scientific conf. «Actual problems of fisheries and aquaculture for basins of the southern seas of Russia». Rostov-on-Don, 1–3 October 2014. (In Russian)

Kuzminova, N. S. 2016. Dependence of alkaline phosphatase activity on bioecological parameters of Black Sea fish. Journal of Ichthyology 56(2):298–303.

Lowry, O. H., Rosbrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry 193:265–275.

Mann, H. B. and Whitney, D. R. 1947. On a test of whether one of 2 random variables is stochastically larger than the other. Annals of Mathematical Statistics 18:50‒60.

Mironov, O. G. and Alemov, S. V. 2018. Sanitary and biological research of coastal water areas of south-western Crimea at the beginning of the XXI century. Institut morskih biologicheskih issledovanij im. A.O. Kovalevskogo RAN, Izdatelstvo IT «AREAL», Simferopol. 276 p. (In Russian)

Moreau, Y., Desseaux, V., Koukiekolo, R., Marchis-Mouren, G., and Santimone, M. 2001. Starch digestion in tropical fishes: isolation, structural studies and inhibition kinetics of alpha-amylases from two tilapias Oreochromis niloticus and Sarotherodon melanotheron. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 128:543‒552.

Munilla-Moran, R., Stark, J. R. 1990. Metabolism in marine flatfish. VI. Effect of nutritional state on digestion in turbot, Scophthalmus maximus (L.). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 95:625‒634.

Oven, L. S., Salekhova, L. P., and Kuz'minova, N. S. 2008. Long-term dynamics of the species composition and abundance of Black Sea fish in the region of Sevastopol. Fisheries in Ukraine 4(57):15‒18. (In Russian)

Pantoja, T. M. A., Rosas, F. C. W., Da Silva, V. M. F., and Dos Santos, A. M. F. 2010. Urinary parameters of Trichechus inunguis (Mammalia, Sirenia): reference values for the Amazonian Manatee. Brazilian Journal of Biology 70(3):607‒615.

Pozdnyakova, I. A., Ivanov, V. V., Kanskaya, N. V., Timin, O. A., and Kolesova, N. I. 2009. Laboratory workshop on biological chemistry for students of Faculty of Pharmacy: Tutorial. Izdatelstvo Siberian State Medical University, Tomsk. 250 p. (In Russian)

Pravdin, I. F. 1966. Guidelines for the study of fish. Izdatelstvo Pishevaya promislennost, Moscow. 374 р. (In Russian)

Rafrafi-Nouira, S., Kamel-Moutalibi, O. El., Boumaїza, M., and Capapé, C. 2016. Food and feeding habits of black scorpionfish, Scorpaena porcus (Osteichthyes: Scorpaenidae) from Northern coast of Tunisia (Central Mediterranean). Journal of Ichthyology 56(1):107‒123.

Revkov, N. K. 2011. Macrozoobenthos of the Ukrainian shelf of the Black Sea; pp. 140–162 in V. N. Eremeev, A. V. Gaevskaya, G. E. Shul'man, Yu. A. Zagorodnyaya (ed.): Promyslovye bioresursy Chernogo i Azovskogo morej. NAN Ukrainy, Institut biologii yuzhnyh morej NAN Ukrainy. Izdatelstvo EKOSI-Gidrofizika, Sevastopol. (In Russian)

Rudneva, I. I., Kuzminova, N. S., Skuratovskaya, E. N., and Kovyrshina, T. B. 2010. Age composition and antioxidant enzyme activities in blood of Black Sea teleosts. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 151(2):229–239.

Sadhu, N., Sharma, S. R. K., Joseph, S., Dube, P., and Philipose, K. K. 2014. Chronic stress due to high stocking density in open sea cage farming induces variation in biochemical and immunological functions in Asian seabass (Lates calcarifer, Bloch). Fish Physiology and Biochemistry 40(4):1105‒1113.

Şevket Kandemir, S., Örün, İ, Talas, Z., Örün, G.N., Erdoğan, K., Işık, M., Altaş, L., and Duran, A. 2010. Effects on mortality of biochemical and limnological properties on some fish species in Sultansuyu Dam Lake (Malatya), Turkey. Turkish Journal of Fisheries and Aquatic Sciences 10:431‒437.

Tizon, R. U., Serrano, A. E., and Traifalgar, Jr. R. F. M. 2013. Influence of size on digestive enzyme activities in the Angelwing clam Pholasorientalis. European Journal of Experimental Biology 3(1):602‒607.

Tonel, F. R., Marini, P., Moraes, C. L., de Magalhães Bandeira, J., Baudet Labbé, L. M., Villela, F. A., and de Moraes, D. M. 2013. Physiological and biochemical changes in seeds and seedlings of redclover submitted to diesel oil. IHERINGIA, Série Botânica, Porto Alegre 68(2):195–201.

Tovar-Ramírez, D., Zambonito Infante, J., Cahu, C., Gatesoupe, F. J., and Vázquez-Juárez, R. 2004. Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development. Aquaculture 234:415–427.

Ugolev, A. M. and Kuzmina, V. V. 1993. Digestive processes and the adaptation of fish. Izdatelstvo Gidrometeoizdat, Saint Petersburg. 120 р. (In Russian)

Vdodovich, I. V. 2008. Nutrition of the larvae of the Black Sea gobies in the coastal waters of Sevastopol in the summer period based on materials from 2003–2009. Ecology of the sea 76:40‒44. (In Russian)

Vinnikova, S. V. 2010. Membrane digestion in rabbits under stress conditions and its correction. Author. Diss. Cand. Thesis. Diagnosis of diseases and therapy of animals, pathology, oncology and morphology of animals. Saint Petersburg. 25 p. (In Russian)

Umalatha, S. N., Kushwaha J. P., and Gangadhar, B. 2016. Digestive enzyme activities in different size groups and segments of the digestive tract in Labeo rohita (Day, 1878). Journal of Aquaculture and Marine Biology 4(5):00098.

Yousefian, M., Sheikholeslami, A., Hedayatifard, M., Dehpour, A. A., Fazli, H., Chiaci, M., Farabi, S. V., and Najafpour, S. H. 2010. Serum biochemical parameters of male and female rainbow trout (Oncorhynchus mykiss) cultured in Haraz River, Iran. World Journal of Island Marine Science 2(6):513–518.

Zacarias-Soto, M., Barón-Sevilla, B., and Lazo, J. P. 2013. Ontogeny and distribution of alkaline and acid phosphatase in the digestive system of California halibut larvae (Paralichthys californicus). Fish Physiology and Biochemistry 39:1331–1339.–013-9787-5

Zavyalov, A. V., Zalevskaya, I. N., Kovirshina, N. V., Kuzminova, N. S., Oven, L. S., Omelchenko, S. O., Poduvay, Y. V., Salekhova, L. P., Samotoy, Y. V., Skuratovskaya, E. N., Chesnokova, I. I., Shaida, V. G., and Shevchenko, N. F. 2016. Ecotoxicological studies of the coastal Black Sea ichthyofauna in the region of Sevastopol. Izdatelstvo GEOS, Moscow. 316 p. (In Russian)

How to Cite
Kuzminova, N. (2020). Variability of the hepatic α-amilase activities in the liver of fish species from the Sevastopol bays, Black Sea. Biological Communications, 64(4), 235–243.
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