Constant darkness conditions modulate the effects of melatonin and luzindole on the antioxidant enzyme activities and levels of retinol and α-tocopherol in rats
DOI:
https://doi.org/10.21638/spbu03.2019.305Abstract
This study was conducted to evaluate the effects of both exogenous melatonin and melatonin receptor antagonist luzindole on the activities of antioxidant enzymes (AOE) (superoxide dismutase, SOD; catalase, CAT) and the level of low-molecular antioxidant vitamins (retinol, α-tocopherol) in male Wistar rats kept in normal light conditions (LD 12:12) or constant darkness (DD). In LD, while melatonin had no influence on the studied antioxidants, luzindole caused an increase in retinol and a decrease in α-tocopherol contents in the liver compared to the control. In DD, with no influence on AOE activities, both drugs exerted similar effects on the liver retinol and kidney α-tocopherol contents, increasing them in comparison with control. Exposing the animals to DD induced an increase in kidney SOD activity and in liver retinol content. Moreover, DD-mel rats had higher SOD activity in the liver and kidney and a higher retinol level in the liver compared to LD-mel ones; DD-luz rats had a higher liver retinol content compared to LD-luz ones. Liver retinol level seems to be the most sensitive to influence of DD, melatonin and luzindole; the data are probably connected with the involvement of vitamin A in the regulation of circadian rhythms.
Keywords:
constant darkness, melatonin, luzindole, antioxidant enzymes, retinol, α-tocopherol
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Published
2019-10-30
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Kalinina, S., Ilyukha, V., Khizhkin, E., Baishnikova, I., Antonova, E., & Morozov, A. (2019). Constant darkness conditions modulate the effects of melatonin and luzindole on the antioxidant enzyme activities and levels of retinol and α-tocopherol in rats. Biological Communications, 64(3), 211–218. https://doi.org/10.21638/spbu03.2019.305
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