Dynamics of physiologically active cells of pico- and nanophytoplankton in the coastal waters of Black Sea

Ekaterina Solomonova

doi:10.21638/spbu03.2016.104

Authors

Ekaterina Solomonova A. O. Kovalevsky Institute of Marine Biological Researches RAS, 2, pr. Nakhimova, Sevastopol, 299011, Crimea, Russian Federation

DOI:

https://doi.org/10.21638/spbu03.2016.104

Abstract

The dynamics of the abundance and viability index (VI) of pico- and nanophytoplankton in the coastal waters of Black Sea near Sevastopol were investigated by regular monthly monitoring using flow cytometry and fluorescein diacetate vital dye (FDA), as a marker of intracellular enzyme activity. Directed seasonal changes in the fraction of physiologically active cells in the study groups of algae in coastal waters was not identified. It is noted that, in the warm season and during vegetative growth of algae in the composition and pico nano-phytoplankton predominate living cells, the percentage of which varies from 70 to 100 %. Low values of the VI (30 %) picophytoplankton were obtained at individual stations in September, October and December, possibly due to the presence of this kind of cell size group Synechococcus, which are very sensitive to fluctuations of environmental factors. Refs 28. Figs 7.

Keywords:

flow cytometry, picophytoplankton, nanophytoplankton, vitality index, fluorescein diacetate

Downloads

Download data is not yet available.

References

Agustí S., Sánchez M. C. Cell viability in natural phytoplankton communities quantified by a membrane permeability probe. Limnol. Oceanogr., 2002, vol. 47, pp. 818–828.

Alonso-Laita P., Agustí S. Contrasting patterns of phytoplankton viability in the subtropical NE Atlantic Ocean. Aquatic microbial ecology, 2006, vol. 43, pp. 67–78.

Kulk G., van de Poll W. H., Visser R. J., Buma A. G. Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton. Limnol. Oceanogr., 2013, vol. 58, pp. 1747–1760.

Lusta K. A., Fikhte B. A. Metody diagnostiki zhivykh kletok po ikh metabolicheskoi aktivnosti [Methods of living cells diagnosis in their metabolic activity]. Metody opredeleniia zhiznesposobnosti mikroorganizmov [Methods for determining of microorganisms viability]. Ed. by V. K. Eroshin. Pushchino, ONTI NTsI AS USSR Publ., 1990, pp. 53–76. (In Russian)

Goriunova S. V. Primenenie metoda fluorestsentnoi mikroskopii dlia opredeleniia zhivykh i mertvykh kletok vodoroslei [The application of fluorescence microscopy for determine the living and dead algae cells]. Vestn. AN SSSR [Bulletin of the Academy of Sciences of the USSR], 1951, vol. 6, pp. 100–102. (In Russian)

Jochem F. Dark survival strategies in marine phytoplankton assessed by cytometric measurement of metabolic activity with fluorescin diacetate. Mar. Biol., 1999, vol. 135, pp. 721–728.

Stauber J. L., Franklin N. M., Adams M. S. Applications of flow cytometry to ecotoxicity testing using microalgae. TRENDS in Biotechnology, 2002, vol. 20, pp. 141–143.

Bentley-Mowat J. A. Application of fluorescence microscopy to pollution studies on marine phytoplankton. Bot. Mar., 1982, vol. 28, pp. 203–204.

Onji M., Sawabe T., Ezura Y. An evaluation of viable staining dyes suitable for marine phytoplankton. Bul. Fac. Fish Hokkaido Univ., 2000, vol. 51, pp. 151–158.

Smith S. R. Flow cytometric analysis of phytoplankton viability in Elkhorn Slough. California. Master’s Theses and Graduate Research, 2009.

Roukhiiainen M. I. Sostav poverkhnostnogo fitoplanktona Sevastopol'skoi bukhty po rezul'tatam liuminestsentnogo analiza [The composition of surface phytoplankton in Sevastopol Bay by the results of luminescence]. Ekosistemy shel'fovykh zon [Ecosystems offshore zones], 1988, pp. 15–38. (In Russian)

Senicheva M. I. Dinamika populiatsii Sceletonema costatum (Grev.) v Sevastopol'skoi bukhte [Population dynamics of Skeletonema costatum (Grev.) in Sevastopol Bay]. Ekologiia moria [Ecology of the sea], 1980, vol. 1, pp. 11–15. (In Russian)

Solomonova E. S., Mukhanov V. S. Otsenka doli fiziologicheski aktivnykh kletok v nakopitel'nykh kul'turakh Phaeodactylum tricornutum i Nitzschia sp. s pomoshch'iu protochnoi tsitometrii [Flow cytometry for the assessment of physiological active cells in batch cultures of Phaeodactylum tricornutum and Nitzschia specia]. MEZh [Sea. Ecol. J.], 2011, vol. 10, pp. 67–72. (In Russian)

Stel'makh L. V., Babich I. I., Liashenko S. V. Sezonnye izmeneniia kontsentratsii khlorofilla “a” i otnosheniia organicheskii uglerod/khlorofill “a” v fitoplanktone pribrezhnykh vod Chernogo moria v raione Sevastopolia po rezul'tatam issledovanii v 2000–2001 gg. [Seasonal changes in the concentration of chlorophyll “a” and the ratio of organic carbon/chlorophyll “a” in the phytoplankton in coastal waters of the Black Sea near Sevastopol on the results of research in 2000–2001]. Ekologiia moria [Sea. Ecol. J.], 2002, vol. 61, pp. 64–68. (In Russian)

Stel'makh L. V., Gubanov V. I., Babich I. I. Sezonnye izmeneniia skorosti rosta i limitirovanie fitoplanktona pitatel'nymi veshchestvami v pribrezhnykh vodakh Chernogo moria v raione Sevastopolia [Seasonal changes in the growth rate and nutrient limitation of phytoplankton in the coastal waters of the Black Sea near Sevastopol]. Morsk. ekol. zhurn. [Sea. Ecol. J.], 2004, vol. 3, no. 4, pp. 55–73. (In Russian)

Chmyr V. D. et al. Strukturno-funktsional'nye parametry planktona chernomorskogo pribrezh'ia [Structural and functional parameters of the coasts of the Black Sea plankton]. Sistemy kontrolia okruzhaiushchei sredy [Environmental control systems], 2007, vol. 13, pp. 335–238. (In Russian)

Stel'makh L. V., Kuftarkova E. A., Akimov I. A., Babich I. I., Kozhemiaka A. B. Ispol'zovanie peremennoi fliuorestsentsii khlorofilla in vivo dlia otsenki funktsional'nogo sostoianiia fitoplanktona [The use of variable chlorophyll fluorescence in vivo to evaluate the functional state of phytoplankton]. Sistema kontrolia okruzhaiushchei sredy [Environmental control systems], 2010, vol. 13, pp. 263–268. (In Russian)

Osadchaia T. S. Kharakteristika razmernoi struktury fitoplanktona po izmereniiam khlorofilla “a” [Characteristics of phytoplankton size structure by measuring chlorophyll “a”]. Ekologiia moria [Ecology of the sea], 2007, vol. 73, pp. 70–74. (In Russian)

Agawin N. S. R., Duarte C. M., Agusti S. Growth and abundance of Synechococcus sp. in a Mediterranean Bay: seasonality and relationship with temperature. Mar. Ecolog. Progress Series, 1998, vol. 170, pp. 45–53.

Labré s. M., Agustí S. Picophytoplankton cell death induced by UV radiation: Evidence for oceanic Atlantic communities. Limnol. Oceanogr., 2006, vol. 51, pp. 21–29.

Agustí S. Viability and niche segregation of Prochlorococcus and Synechococcus cells across the Central Atlantic Ocean. Aquatic microbial ecology, 2004, vol. 36, pp. 53–59.

Gechev T. S., Van Breusegem F., Stone J. M., Denev I., Laloi C. Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bioessays, 2006, vol. 28, pp. 1091–1101.

Raven J. A., Giordano M., Beardall J., Maberly S. C. Algal and aquatic plant carbon concentrating mechanisms in relation to environmental change. Photosynthesis Research, 2011, vol. 109, pp. 281–296.

Kalff J., Knoechel R. Phytoplankton and their dynamics in oligotrophic and eutrophic lakes. An. Review of Ecology and Systematics, 1978, pp. 475–495.

Goriunova S. V. Primenenie metoda fluorestsentnoi mikroskopii dlia opredeleniia zhivykh i mertvykh kletok vodoroslei [Application of fluorescence microscopy to determine the living and the dead algae cells]. Tr. In-ta mikrobiol. [Proceedings of the Institute of Microbiology], 1952, vol. 2, pp. 64–57. (In Russian)

Kuftarkova E. A., Eremin I. Iu., Subbotin A. A. Tonkaia vertikal'naia khimicheskaia struktura vod v raione midiinoi fermy (predprolivnaia chast' Sevastopol'skoi bukhty) [Thin vertical chemical structure of water in the area mussel farm (Sevastopol Bay)]. MEZh [Sea. Ecol. J.], 2011, vol. 10, pp. 38–42. (In Russian)

Solomonova E. S. Opredelenie potentsial'noi skorosti rosta fitoplanktona s ispol'zovaniem mitoticheskogo indeksa i protochnoi tsitometrii [Determination of the potential growth rate of phytoplankton using the mitotic index and flow cytometry]. Tezisy 7-i mezhdunarodnoi nauchno-prakticheskoi konferentsii molodykh uchenykh “Pont Evksinskii — 2011” (Sevastopol', 2011) [Abstracts of 7th International Scientific and Practical Conference of Young Scientists “Pontus Euxine — 2011” (Sevastopol, 2011)]. Sevastopol', EKOSIGidrofizika Publ., 2011, pp. 220–222. (In Russian)

Hayakawa M., Suzuki K., Saito H., Takahashi K., Ito S. I. Differences in cell viabilities of phytoplankton between spring and late summer in the northwest Pacific Ocean. J. of Exper. Mar. Biol. and Ecol., 2008, vol. 360, no. 2, pp. 63–70.