Molecular cytogenetic characterization of two murine cancer cell lines derived from salivary gland

Ralf Steinacker; Thomas Liehr; Nadezda Kosyakova; Martina Rincic; Shaymaa S. Hussein Azawi

doi:10.21638/spbu03.2018.403

Authors

Ralf Steinacker Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Am Klinikum 1, D-07747 Jena, Germany
Thomas Liehr Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Am Klinikum 1, D-07747 Jena, Germany https://orcid.org/0000-0003-1672-3054
Nadezda Kosyakova Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Am Klinikum 1, D-07747 Jena, Germany
Martina Rincic Department for Functional Genomics, Centre for Translational and Clinical Research, University Hospital Centre Zagreb, University of Zagreb School of Medicine, Zagreb, Croatia
Shaymaa S. Hussein Azawi Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Am Klinikum 1, D-07747 Jena, Germany

DOI:

https://doi.org/10.21638/spbu03.2018.403

Abstract

Here two murine salivary gland cancer (SGC) cell lines WR21 and SCA-9 were studied for the first time in detail by high-resolution molecular cytogenetic approaches. This study revealed that these cell lines are models for human SGCs of initial stage myoepithelioid or mucoepidermoid (WR21) and of advanced stage mucoepidermoid (SCA-9) tumors. Besides, three genes most likely playing a role in SGC development (FGF10, ELAVL1/HUR and SEL1) were identified. All of them were involved in translocation events in these in vitro models and thus were most likely activated. Overall, the present study highlights the necessity not only to establish but also to genetically characterize murine tumor cell lines. Without such a characterization they cannot be used in a reasonable way in research.

Keywords:

salivary gland cancer (SGC), murine tumor cell lines, WR21, SCA-9, myoepithelioid SGC, mucoepidermoid SGC, FGF10, ELAVL1/HUR, SEL1

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References

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