The preparation of samples for studying neutrophils without their isolation
DOI:
https://doi.org/10.21638/spbu03.2023.302Abstract
Neutrophils are the primary cells of the innate immune system. They destroy pathogenic microorganisms carrying out the oxidative stress mechanism through phagocytosis and extracellular traps (NETs). There is no doubt about the need to study neutrophils’ functional activity, but there are some methodological problems with the nativity of conditions in such experiments. The aim of this work is to propose a method of preparing a sample of peripheral blood neutrophils for study by spectral-optical methods (such as confocal microscopy) providing an increased level of the nativity of the conditions.
Keywords:
neutrophils, reactive oxygen species, respiratory burst, confocal microscopy
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References
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Published
2023-11-30
How to Cite
Fedorova, N., Sokolov, A., Trashkov, A., & Varfolomeeva, E. (2023). The preparation of samples for studying neutrophils without their isolation. Biological Communications, 68(3), 145–150. https://doi.org/10.21638/spbu03.2023.302
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Articles of Biological Communications are open access distributed under the terms of the License Agreement with Saint Petersburg State University, which permits to the authors unrestricted distribution and self-archiving free of charge.
