Multiparametric comparative analysis of coelomocytes in <em>Asterias amurensis</em> and <em>Lysastrosoma anthosticta</em>

Yuriy Karetin; Eugenia Pimenova

doi:10.21638/spbu03.2018.304

Authors

Yuriy Karetin A. V. Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Palchevskogo Str, 17, Vladivostok, 690041, Russian Federation https://orcid.org/0000-0002-0760-6721
Eugenia Pimenova A. V. Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Palchevskogo Str, 17, Vladivostok, 690041, Russian Federation

DOI:

https://doi.org/10.21638/spbu03.2018.304

Abstract

Behavioral dynamics of coelomocytes from echinoderms Asterias amurensis and Lysastrosoma anthosticta during the first hour of in vitro cultivation was analyzed using a wide range of linear and fractal parameters of the external morphology. Most of the parameters, including values of the cell bounding circle and convex hull, asymmetry, fractal dimensions of contour images, lacunarity, and cell density and area, showed species-specific behavior of the immune cells of the studied animals. The cells differed in a wide range of parameters as early as two min after seeding on the substrate. The cells acquired the largest morphological differences by the fifth min of cultivation. Both cell dynamics in general and analysis of the cell morphology at individual points in time may serve as markers for species-specificity of cells. However, when morphology is compared at one point in time, at least two parameters associated with significant morphological differences in cells of the studied species should be used because of overlapping tendencies in changes in morphological features.

Keywords:

Asterias amurensis, Lysastrosoma anthosticta, coelomocytes, morphometry, fractal analysis, cell morphology

Downloads

Download data is not yet available.

References

Adema, C. M., Harris, R. A., and van Deutekom-Mulder, E. C. 1992. A comparative study of hemocytes from six different snails: Morphology and functional aspects. Journal of Invertebrate Pathology 59:24−32. https://doi.org/10.1016/0022-2011(92)90107-F

Borges, R. M., Lamers, M. L., Forti, F. L., Santos, M. F., and Yan C. Y. 2011. Rho signaling pathway and apical constriction in the early lens placode. Genesis 49:368−379. https://doi.org/10.1002/dvg.20723

Chauhan, B. K., Lou, M., Zheng, Y., and Lang, R. A. 2011. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proceedings of the National Academy of Sciences, U S A 108(45):18289−18294. https://doi.org/10.1073/pnas.1108993108

Chen, J. H., and Bayne, C. J. 1995. Hemocyte adhesion in the California mussel (Mytilus californianus): regulation by adenosine. Biochimica et Biophysica Acta ‒ Molecular Cell Research 1268:178–184. https://doi.org/10.1016/0167-4889(95)00074-3

Cheng, T. C. (Ed.). 1984. Comparative Pathobiology. Invertebrate Blood. Vol. 6. Springer Science+Business Media. New York. https://doi.org/10.1007/978-1-4684-4766-8

Chernyavskikh, S. D., Fedorova, M. Z., Thanh, V. V., and Quyet, D. H. 2012. Reorganization of actin cytoskeleton of nuclear erythrocytes and leukocytes in fish, frogs, and birds during migration. Cell and Tissue Biology 6:348‒352. https://doi.org/10.1134/S1990519X12040025

Dolatshahi-Pirouz, A., Kolind, J. K., Bünger, C., Kassemd, M., Foss, M., and Besenbacher, F. 2011. Cell shape and spreading of stromal (mesenchymal) stem cells cultured on ﬁbronectin coated gold and hydroxyapatite surfaces. Colloids and Surfaces B: Biointerfaces 84:18−25. https://doi.org/10.1016/j.colsurfb.2010.12.004

Domínguez-Giménez, P., Brown, N. H., and Martín-Bermudo, M. D. 2007. Integrin-ECM interactions regulate the changes in cell shape driving the morphogenesis of the Drosophila wing epithelium. Journal of Cell Science 120:1061−1071. https://doi.org/10.1242/jcs.03404

Dyrynda, E. A., Pipe, R. K., and Ratcliffe, N. A. 1997. Sub-populations of haemocytes in the adult and developing marine mussel, Mytilus edulis, identified by use of monoclonal antibodies. Cell and Tissue Research 289:527‒536. https://doi.org/10.1007/s004410050898

Fisher, W. S. 1986. Structure and functions of oyster hemocytes; pp. 25‒35 in Immunity in invertebrates. M. Brehelin, J. M. Arcier, N. Boemare, J. R. Bonami, C. P. Vivares, (Eds.) Berlin; Heidelberg: Springer-Verlag. https://doi.org/10.1007/978-3-642-70768-1_3

Gordon, A. D. 1999. Classification. Boca Raton, FL: Chapman and Hall, CRC.

Hine, P. M. 1999. The inter-relationships of bivalve haemocytes. Fish Shellfish Immunology 9:367‒385. https://doi.org/10.1006/fsim.1998.0205

Kalitnik, A. A., Karetin, Y. A., Kravchenko, A. O., Khasina, E. I., and Yermak, I. M. 2017. Influence of carrageenan on cytokine production and cellular activity of mouse peritoneal macrophages and its effect on experimental endotoxemia. Journal of Biomedical Materials Research Part A 105:1549−1557. https://doi.org/10.1002/jbm.a.36015

Karetin, Y. A., and Pushchin, I. I. 2015. Analysis of the shapes of hemocytes of Callista brevisiphonata in vitro (Bivalvia, Veneridae). Cytometry A 87:773−776. https://doi.org/10.1002/cyto.a.22676

Karetin, Y. A. 2016. Nonlinear analysis of hemocyte morphology in the sea stars Aphelasterias japonica (Bell, 1881), Patiria pectinifera (Muller et Troschel, 1842), and the bivalve Callista brevisiphonata (Carpenter, 1864). Russian Journal of Marine Biology 42:275−282. https://doi.org/10.1134/S1063074016040052

Karetin, Y. and Pushchin, I. 2017. Analysis of the shapes of coelomocytes of Aphelasterias japonica in vitro (Echinodermata: Asteroidea). Protoplasma 254:1805−1811. https://doi.org/10.1007/s00709-017-1078-z

Kolyuchkina, G. A. and Ismailov, A. D. 2011. Morphofunctional features of bivalve mollusks during experimental contamination of the medium with heavy metals. Oceanology 51:804‒813. https://doi.org/10.1134/S0001437011050092

Mermelstein, C. S., Rebello, M. I., Amaral, L. M., and Costa, M. L. 2003. Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca²⁺ chelation. Brazilian Journal of Medical and Biological Research 36:1111−1116. https://doi.org/10.1590/S0100-879X2003000800018

Paine, R. 3rd, Christensen, P., Toews, G. B., and Simon, R. H. 1994. Regulation of alveolar epithelial cell ICAM-1 expression by cell shape and cell-cell interactions. American Journal of Physiology 266:L476−L484. https://doi.org/10.1152/ajplung.1994.266.4.L476

Perez, D. G. and C. S. Fontanetti. 2011. Hemocitical responses to environmental stress in invertebrates: a review. Environmental Monitoring and Assessment 177:437‒447. https://doi.org/10.1007/s10661-010-1645-7

Ratner, S. and Vinson, S. B. 1983. Phagocytosis and encapsulation: cellular immune responses in Arthropoda. American Zoologist 23:185−194. https://doi.org/10.1093/icb/23.1.185

Rioult, D., Lebel, J.-M., and Le Foll, F. 2013. Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (Mytilus edulis) hemocytes in vitro. Cytotechnology 65:749‒758. https://doi.org/10.1007/s10616-013-9558-2

Schweitzer, L. and Renehan, W. E. 1997. The use of cluster analysis for cell typing. Brain Research Protocols 1:100−108. https://doi.org/10.1016/s1385-299x(96)00014-1

Vignaud, T., Galland, R., Tseng, Q., Blanchoin, L., Colombelli, J., and Théry, M. 2012. Reprogramming cell shape with laser nano-patterning. Journal of Cell Science 125:2134−2140. https://doi.org/10.1242/jcs.104901

Welsh, G. I. and Saleem M. A. 2012. The podocyte cytoskeleton ― key to a functioning glomerulus in health and disease. Nature Reviews Nephrology 8:14−21. https://doi.org/10.1038/nrneph.2011.151