Microencapsulation contaning L. acidophilus and S. boulardii for targeted release in the stomach and small intestine
DOI:
https://doi.org/10.21638/spbu03.2024.203Abstract
In this study, a microencapsulation system was developed with a dual-layer structure comprising Lactobacillus acidophilus and Saccharomyces boulardii to enhance the survival rates of probiotic bacteria during transit through the stomach. Alginate and chitosan were identified as key factors influencing the shape, moisture content, and the number of encapsulated microorganisms in the microcapsules. The optimal concentrations of alginate and chitosan were determined to be 0.5 % and 3 %, respectively. The microencapsulated structure was clearly visualized through Scanning Electron Microscope images, and Infrared spectra confirmed the successful encapsulation. Experimental findings revealed that S. boulardii was released directly in the stomach, while L. acidophilus was released after a 2-hour delay, coinciding with the arrival of microcapsules in the small intestine. Subsequently, we proposed the release kinetics of microcapsules in the gastrointestinal tract. Although the viability of both S. boulardii and L. acidophilus in the microcapsules exhibited a steady decline over the storage period, a notable 109 CFU/g of bacteria persisted even after 120 days.
Keywords:
probiotics, L. acidophilus, S. boulardii, viability, release
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Published
2024-10-10
How to Cite
Ngo, A., Minh, T., Bui, N., & Dam, X. (2024). Microencapsulation contaning <em>L. acidophilus</em> and <em>S. boulardii</em> for targeted release in the stomach and small intestine. Biological Communications, 69(2), 76–89. https://doi.org/10.21638/spbu03.2024.203
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