Ethanolic extract of <em>Aloe arborescens</em> stimulates neonatal rat calvarial cells proliferation, migration and osteogenic differentiation

Nicolás Olegario Blanco; Valeria Gili; Juan Andrés Laiuppa; Graciela Edith Santillán

doi:10.21638/spbu03.2023.303

Authors

Nicolás Olegario Blanco Department of Biology, Biochemistry and Pharmacy of the National University of the South, INBIOSUR-CONICET, San Juan, 670, B8000ICN, Bahía Blanca, Argentina https://orcid.org/0009-0009-7863-9916
Valeria Gili Department of Biology, Biochemistry and Pharmacy of the National University of the South, INBIOSUR-CONICET, San Juan, 670, B8000ICN, Bahía Blanca, Argentina
Juan Andrés Laiuppa Department of Biology, Biochemistry and Pharmacy of the National University of the South, INBIOSUR-CONICET, San Juan, 670, B8000ICN, Bahía Blanca, Argentina
Graciela Edith Santillán Department of Biology, Biochemistry and Pharmacy of the National University of the South, INBIOSUR-CONICET, San Juan, 670, B8000ICN, Bahía Blanca, Argentina https://orcid.org/0000-0001-6165-6781

DOI:

https://doi.org/10.21638/spbu03.2023.303

Abstract

The medicinal plant Aloe arborescens Miller has chemical compounds that could stimulate the activity of bone-forming cells, but no studies have been found in this regard. We evaluated the effects of different dilutions of aqueous (1/10; 1/100; 1/1000) or ethanolic (1/1000; 1/2000; 1/5000) extracts of parenchyma from A. arborescens on the viability, proliferation, migration and osteogenic differentiation of primary cell cultures from neonatal rat calvaria. In none of the conditions studied did cell viability decrease (p ≤ 0.01). Furthermore, the 1/5000 ethanolic extract dilution showed a positive effect on cell viability at 48 h and 72 h and the latter was correlated with a 27 % (p ≤ 0.01) increase in cell proliferation. Ethanolic extract significantly stimulated cell migration and cultured mineralization with respect to control, showing the maximal effect at a dilution 1/5000. Together, the results show that the A. arborescens extracts do not have toxic effects. In addition, ethanolic extract stimulates proliferation, migration and osteogenic differentiation of rat calvarial cells, suggesting a potential bone anabolic action.

Keywords:

osteoblast, proliferation, migration, osteogenic differentiation

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References

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