TY - JOUR AU - Karlov, Denis AU - Sazanova, Anna AU - Kuznetsova, Irina AU - Tikhomirova, Nina AU - Popova, Zhanna AU - Osledkin, Yuriy AU - Demidov, Nikita AU - Belimov, Andrey AU - Safronova, Vera PY - 2021/03/31 Y2 - 2024/03/28 TI - Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic JF - Biological Communications JA - BioComm VL - 66 IS - 1 SE - Full communications DO - 10.21638/spbu03.2021.109 UR - https://biocomm.spbu.ru/article/view/8769 SP - 73–82 AB - <p>Twenty-nine strains were isolated from two samples of the permafrost active layer of the Spitsbergen archipelago. The estimated number of bacteria ranged from 4.0⋅10<sup>4</sup> to 1.7⋅10<sup>7</sup> CFU∙g<sup>-1</sup>. As a result of sequencing of the 16S rRNA (rrs) genes, the isolates were assigned to 13 genera belonging to the phyla Actinobacteria, Proteobacteria (classes α, β, and γ), Bacteroidetes, and Firmicutes. Six isolates belonged to the rhizobial genus <em>Mesorhizobium</em> (order Rhizobiales). A plant nodulation assay with seedlings of legume plants <em>Astragalus norvegicus, A. frigidus, A. subpolaris</em> and <em>Oxytropis sordida</em>, originated from Khibiny (Murmansk region, Russia) and inoculated with <em>Mesorhizobium</em> isolates, showed the inability of these strains to form nodules on plant roots. Symbiotic (<em>sym</em>) genes <em>nodC</em> and <em>nodD</em> were not detected in <em>Mesorhizobium</em> strains either.</p> ER -