@article{Onishchuk_Kurchak_Chizhevskaya_Simarov_Provorov_2021, title={Supply of acetyl-CoA to N<sub>2</sub>-fixing bacteroids: insights from the mutational and proteomic analyses of <em>Sinorhizobium meliloti</em>}, volume={66}, url={https://biocomm.spbu.ru/article/view/9265}, DOI={10.21638/spbu03.2021.203}, abstractNote={&lt;p&gt;Rhizobia represent a diverse group of gram-negative bacteria capable of fixing atmospheric nitrogen in symbiosis with leguminous plants. Mechanisms of symbiotic efficiency are important to study not only to reveal the “fine tuning” of the host–symbiont supra-organismal genetic system emergence, but also to develop agriculture with minimal environmental risks. In this paper we demonstrate that among seven genes whose inactivation by Tn5 insertions results in an increased efficiency of rhizobia (&lt;em&gt;Sinorhizobium meliloti&lt;/em&gt;) symbiosis with alfalfa (Eff&lt;sup&gt;++&lt;/sup&gt; phenotype), six genes are involved in the metabolism of small molecules. One of them (SMc04399) encodes for acetate-CoA transferase catalyzing the formation of acetyl-CoA from acyl-CoA. Since acetyl-CoA is required for operation of the Krebs cycle, providing ATP for symbiotic N&lt;sub&gt;2&lt;/sub&gt; fixation, we suggest that a significant portion of this coenzyme utilized by bacteroids is provided by the plant cell supporting the energy-consuming nitrogenase reaction. Proteomic data analysis allow us to reveal the lability of enzymatic pathways which are involved in bacteroids in the production and catabolism of acetyl-CoA and which should be modified to obtain the Eff&lt;sup&gt;++&lt;/sup&gt; phenotype. This phenotype was developed also after inactivation of NoeB protein which is involved in the host-specific nodulation and is characterized by an elevated production in wild type &lt;em&gt;S. meliloti &lt;/em&gt;bacteroids, suggesting a multifunctional role of &lt;em&gt;noe&lt;/em&gt;B in the symbiosis operation.&lt;/p&gt;}, number={2}, journal={Biological Communications}, author={Onishchuk, Olga and Kurchak, Oxana and Chizhevskaya, Elena and Simarov, Boris and Provorov, Nikolay}, year={2021}, month={Jun.}, pages={124–128} }